Indirect immunoenzyme assay to test antibodies against dengue virus in human serum/plasma.
- Serum dilution and sorbent treatment, when necessary, directly in the well
- The M1018 reference is based on µ-Capture technique
- Suitable for automated ELISA systems
Indirect Method - The ELISA method is based upon the reaction of antibodies in the sample tested with the antigen adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. An enzyme-labelled anti-human globulin binds the antigenantibody complex in a second step. After a new washing step, bound conjugate is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.
Capture Method - The ELISA method is based upon the capture of IgM in the sample with anti-IgM antibodies adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. Then the antigen labeled with peroxidase react with the IgM captured, and the unbound is eliminated by washing; bound antigen is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.
- High performance and guaranteed stability with lyophilized conjugates when necessary.
- Samples and controls are equally processed to compensate pipetting variability.
- Colour-coded plates with individual break-apart wells.
- Coloured, ready-to-use liquid reagents.
Indirect Method - The ELISA method is based upon the reaction of antibodies in the sample tested with the antigen adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. An enzyme-labelled anti-human globulin binds the antigenantibody complex in a second step. After a new washing step, bound conjugate is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.
Capture Method - The ELISA method is based upon the capture of IgM in the sample with anti-IgM antibodies adsorbed on the polystyrene surface. Unbound immunoglobulins are washed off. Then the antigen labeled with peroxidase react with the IgM captured, and the unbound is eliminated by washing; bound antigen is developed with the aid of a substrate solution (TMB) to render a blue coloured soluble product which turns into yellow after adding the acid stopping solution.
- High performance and guaranteed stability with lyophilized conjugates when necessary.
- Samples and controls are equally processed to compensate pipetting variability.
- Colour-coded plates with individual break-apart wells.
- Coloured, ready-to-use liquid reagents.